About the Facility
FEI Tecnai F30 G2 Twin Microscope
FEI Tecnai F30 Twin is a 300kV field emission gun (FEG) high resolution TEM/STEM which was installed in May 2012. The microscope has a Twin objective lens with a Cs of 2.0 mm. With its 300 kV acceleration voltage and its Schottky field emission source, this instrument provides outstanding stability, ease-of-use and high-resolution performance in TEM/STEM imaging and microanalysis.
The scope is equipped with an Oxford ultra-thin window EDX detector, a Gatan ultra-scan 4000 CCD and Fischione high angle annular dark field detectors for STEM imaging, Quantum Gatan Imaging Filter with 2Kx2K CCD for performing EELS and energy filtered imaging, Fischione tomography holder, Xplore3D Xpress tomography data collection software (batch tomography), ResolveRT batch processing tomography software and AMIRA 3D rendering and segmentation software, Gatan 626 cryo-holder with α +/- 70 º tilt range and ability to keep samples at -170 ºC for ~4 hours, Gatan pumping station, Gatan cryo-blades, low-dose data collection package, Mark IV Vitrobot for making vitreous plunge-frozen samples.
(Left from White, 2010 PLoS) 2D Projection Images of SIV in vitreous ice. (Middle) The B. stearothermophilus pyruvate dehydrogenase E2 core domain. (Right) Model of pyruvate dehydrogenase complex.
(X-ray Energy Dispersive Spectroscopy)
(Left from Holman, 2008 PNAS) Cryo-EM of Desulfovibrio containing electron dense material. (Middle) Elemental composition of electron dense using XEDS. (Right) Experimental display of locations of various elements during XEDS.
(Left from White, 2010 PLoS) Tomographic reconstruction of SIV virion. (Middle) Segmentation of tomographic reconstruction. (Right) 3D classification and averaging of 3D SIV Envelope glycoprotein subvolumes
(Left from T. Gonen, 2004 JMB) Crystals of Aquaporin-0 and electron diffraction patterns of untilted double-layered AQP0 2D crystals typically showed diffraction spots to a resolution better than 3.5 Angstrom (Middle). (Right) Model of Aquaporin.
(Scanning Transmission Electron Microscopy)
(Left from A. Herzing 2008 Science) High-magnification aberration-corrected STEM-HAADF images of (A and B) the inactive and (C and D) the active Au/FeOx catalysts acquired with STEM. (Right from Sousa, 2011 JSB) Axial STEM tomogram showing a 1-μm-thick section of pancreatic beta cells.
(Electron Energy Loss Spectroscopy)