MU Stacked LogoElectron Microscopy Core Facility, University of Missouri
Contact Us

If you would like learn how to operate our microscopes OR to take a course on electron microscopy, please also see our Training link.

Physics 7230/4320 "Electron Microscopy and Microanalysis"
The Electron Microscopy Core Facility's premier course for understanding theory behind and operation of electron microscopes. Lectures contain theory behind imaging and microanalysis with both scanning and transmission electron microscopes, laboratories demonstrate theory by hands-on operation of scanning (5 labs) and transmission (3 labs) electron microscopes ulitizing various imaging modes (high resolution imaging, secondary & backscattered imaging, variable pressure SEM, energy filtered imaging, STEM) with focus on microanalysis including X-ray energy dispersive spectroscopy (EDS), electron energy loss spectroscopy (EELS), energy filtered imaging (EFTEM), high angle annular dark field scanning transmission electron microscopy (HAADF STEM). Students will propose, perform and present novel imaging and microanalysis related to their research.
2015 syllabus
Lab 1. Introduction to Hitachi S4700 SEM
Lab 2. Secondary and Backscattered Electron Signals
Lab 3. High Resolution SEM
Lab 4. Low Vacuum Mode in Environmental SEM
Lab 5. X-ray Energy Dispersive Spectroscopy for SEM
Lab 6. Introduction to TEM; Direct Alignments and HRTEM imaging
Lab 7. Scanning Transmission Electron Microscopy and X-ray Energy Dispersive Spectroscopy
Lab 8. Energy Filtered Imaging and Spectroscopy

Nanoscale Imaging and Analysis Workshop
Over 4 days, participants in the "Nanoscale Imaging and Analysis" workshop will receive in-depth theory related to transmission electron microscopy and related analytical components available on the state's most powerful transmission electron microscope (FEI Tecnai F30 G2 Twin). Material covered includes components, alignment and operation, image formation, high resolution TEM (HRTEM), high angle annular dark field scanning TEM (HAADF STEM), X-ray energy dispersive spectroscopy (XEDS), electron energy loss spectroscopy (EELS) and energy filtered imaging (EFTEM). After each morning's lecture (~2 hours), students are paired and guided through hands-on operation to demonstrate the morning lecture's theory (~2 hours) for a total of > 6 hours. The final day is reserved for students to perform individual imaging and microanalysis on their own specimens.

Veterinary Pathobiology 8452 "Cellular Light and Electron Microscopy"
2015 EMC demos and deadlines

Biochemistry 9001 "Protein Structure Determination by Electron Microscopy"
This course will introduce and familiarize students with the steps required to obtain 3D information related to protein structure. Included is general TEM operation, introduction to negative staining and variables affecting results, introduction to image processing, 2D & 3D alignment, classification and averaging, plunge freezing vitreous protein samples, and collecting cryo-TEM data. The class culminates with students applying their new found knowledge to their proteins of interest with 2-3 weeks for individual projects. Students gain up to 15 hours of TEM time throughout the course.
2015 syllabus
2015 Lectures
2015 Labs